Fig. 2.

Phosphorylation of Cbp in Csk-deficient embryonic fibroblasts. (A) Raft fractions were prepared from wild-type cells (lane 1), Csk-deficient cells (lane 2), Csk-deficient cells transiently transfected with Csk cDNA (lane 3), and double mutants for Csk and Fyn (lane 4), and immunoblotted with anti-Cbp (Top), anti-Csk (Middle), and anti-Fyn (Lower) antibodies. (B) The raft fractions were probed with an anti-phosphotyrosine (pY) antibody. Positions of molecular mass markers are shown on the left of the blots. (C) Non-raft fractions were probed with an anti-pY antibody.