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. Author manuscript; available in PMC: 2011 Sep 1.
Published in final edited form as: Cytoskeleton (Hoboken). 2010 Sep;67(9):586–598. doi: 10.1002/cm.20469

Figure 1.

Figure 1

The Toxoplasma 49.m03355 gene encodes a small, low complexity protein that localizes to an apical ring. (Top) Clustal alignment of the amino acid sequences of Toxoplasma 49.m03355 (TgRNG1), Neospora (NcRNG1) and Sarcocystis (SnRNG1) homologs. These low complexity proteins are nearly 25% proline. Note that the Sarcocystis homolog has a three residue deletion, a five residue insertion and sequence divergence in regions of the protein that are of higher complexity (i.e. not polyproline stretches). (Bottom, left) A Toxoplasma line with a stable fusion of the endogenous 49.m03355 (RNG1) gene to YFP (green) has a small fluorescent apical ring. (Bottom, right) Tachyzoites expressing a stable fusion of the endogenous 49.m03355 (RNG1) gene to mCherry (red) also show apical ring localization. DNA stained with DAPI is blue. Scale bar = 2 μm.