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. Author manuscript; available in PMC: 2011 Nov 1.
Published in final edited form as: Cytoskeleton (Hoboken). 2010 Nov;67(11):715–728. doi: 10.1002/cm.20482

Figure 2. KLP67A depletion affects the dynamics of growing MT plus-ends.

Figure 2

(A) Comparison of representative EB1-GFP kymographs from control and “mid anti-KLP67A” injected embryos showing that KLP67A depletion allows more growing MTs to cross the spindle equator and enter the distal half spindle to reach the opposite pole. (B) Cartoon showing change in spindle MT organization after KLP67A inhibition. MTs are shown in green and centrosomes are shown in black. Dotted black line shows the arbitrary division of the spindle into three domains along the pole-pole axis which were used to semi-quantify the extent of penetration of one half spindle by MT plus-ends from the other half spindle. (C) Comparison of the percentage of growing MT plus ends that penetrate into the distal one-third spindle region, adjacent to the distal pole (division into the three regions as shown in B) - control and “mid” anti-KLP67A injected embryos are shown. (D) Histogram of EB1 speckle run lengths in control and “mid” anti-KLP67A injected embryos. Average length and total counts for C and D are shown in Table 1.