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. Author manuscript; available in PMC: 2011 Nov 1.
Published in final edited form as: Cytoskeleton (Hoboken). 2010 Nov;67(11):715–728. doi: 10.1002/cm.20482

Figure 4. Higher anaphase B spindle elongation rate after KLP67A depletion is correlated with an increase in the MT sliding rate.

Figure 4

(A) A still image from a time lapse video of a representative embryo injected with anti-KLP67A antibody (at the upper left corner) showing a gradient of mitotic defects. (B) Spindle pole dynamics of the four spindles shown in A compared to controls (WT) showing that KLP67A inhibition caused an increase in anaphase B spindle elongation rate. (C). Representative tubulin kymograph from an embryo partially depleted of KLP67A, showing that tubulin speckles move away from the spindle equator at a similar rate to the rate of spindle pole separation during anaphase B. (D) Linear correlation between the MT sliding rate and anaphase B spindle elongation rate in spindles. Data from two representative embryos displaying a higher anaphase B spindle elongation rate after partial KLP67A depletion compared to a control embryo. P-value=0.0005.R square=0.82. For each spindle, 50-200 speckles were measured.