Fig. 1.

Immunocytochemical detection of neural gene expression in a subset of adult mouse whole ex vivo bone marrow (see Materials and Methods). Double immunocytochemical detection of neurofilament H and Pax-6 was performed in the same subset of bone marrow cells, and expression of neuronal neurofilament H, NeuN, and HuC/HuD was present in a subset of CD34⁺ bone marrow cells. GAD65, an enzyme responsible for synthesis of a major neurotransmitter, also was present in a subset of bone marrow cells. Oligodendroglial CNPase was present in a subset of bone marrow cells, whereas the astroglial marker GFAP was not detected on ex vivo bone marrow. Neurofilament H and Oct-4 were found in the same subset of ex vivo bone marrow cells. DAPI stains of the nuclei of all cells are shown.