Fig. 4.

Detection of RT-PCR products of mRNA from a pure population of CD34⁺ cells derived from adult C57BL/6J bone marrow after 6 weeks in culture. The first lane of each panel shows a 500-bp DNA ladder. (Left) GATA-2 and LMO-2 are hematopoietic stem cell transcription factors. TAL-1 is a lymphocyte transcription factor. (Right) CD34 is a marker of hematopoietic stem cells. FLK-2 is a tyrosine kinase receptor present in hematopoietic stem cells with short-term multilineage reconstitution potential but not long-term potential. GFAP is a marker of differentiated astroglial cells. Rex-1 is a transcription factor in early embryonic stem cells. Equal quantities of RNA were used for each lane from a common pool of CD34⁺ cells in culture. CD34 was used as a positive control.