Table 1. Hematopoietic markers in C57B1/6J mouse bone marrow cells cultured in IL-3, IL-6, and stem cell factor.
| % positive cells
|
|||
|---|---|---|---|
| Marker | Week 3 | Week 4 | Week 16 |
| Hematopoietic stem cells | |||
| CD34 | 95-99 | 100* | 100 |
| Sca-1 | 95-99 | 100 | 100 |
| AA4.1 | 95-99 | 100 | 100 |
| cKit | 95-99 | 100 | 100 |
| All hematopoietic cells | |||
| CD45 | 100 | 100 | 100 |
| HMBP | 100 | 100 | 100 |
| Macrophages | |||
| F4/80 | 1-3 | 0† | 0 |
| Endothelial cells | |||
| Factor 8 | 0 | 0 | ND |
| B cells | |||
| CD19 | 0 | 0 | ND |
| T cells | |||
| CD4 | 0 | 0 | ND |
| CD8 | 0 | 0 | ND |
ND, not determined.
*
All of the analyzed cells were positive.
†
None of the analyzed cells were positive.