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. Author manuscript; available in PMC: 2011 Dec 1.
Published in final edited form as: Microbes Infect. 2010 Oct 1;12(14-15):1244–1251. doi: 10.1016/j.micinf.2010.09.010

Figure 4.

Figure 4

Effect of iron deprivation or supplementation on E. cuniculi replication in murine peritoneal macrophages in vitro. PEC macrophages from WT mice treated with medium (filled bars) or activated with IFNγ plus LPS (open bars) were inoculated with E. cuniculi and various concentrations of the iron chelator, desferoxamine (DFO, Panel A), ferric citrate iron supplement (Panel B), or combination of both (Panel C). Three days later (72 hrs), the cultures were fixed, stained with Calcofluor White, and the numbers of E. cuniculi per 100 macrophages were counted. ANOVA was used to compare infectivity in treated groups of macrophages in comparison to medium-treated (^) or activated (*) macrophages as described in Fig. 1.