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. Author manuscript; available in PMC: 2011 Dec 1.
Published in final edited form as: Mol Ecol. 2010 Nov 3;19(24):5332–5344. doi: 10.1111/j.1365-294X.2010.04888.x

Fig. 5. PCR and Sanger sequencing assessment of SNP identification accuracy.

Fig. 5

Two selected sequence chromatograms from PCR and Sanger sequencing validation of SNPs using blood DNA from Flint, with a summary of DNA capture/GAII sequence data for the variable and adjacent sites from the chromatograms. In total, 50 SNPs were identified from 35,481 bp of chromosome 21 Sanger sequencing data. In the corresponding GAII sequencing data, there were 0 false positive SNPs, and 1 false-negative SNP in each of the blood and fecal DNA results (for different SNPs).