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. Author manuscript; available in PMC: 2012 Sep 1.
Published in final edited form as: Dysphagia. 2010 Aug 31;26(3):256–263. doi: 10.1007/s00455-010-9297-x

Figure 2.

Figure 2

Identification of myosin heavy chain (MHC) isoforms with Western blotting technique in the genioglossus (GG) and soleus muscle of the rat. The soleus, a muscle composed of predominantly type I MHC, is provided as a control to demonstrate accuracy of identification of type I MHC. In the first lane from the left in the panel, MHC isoforms (IIa and I) from a protein stained gel from the soleus muscle. In the second lane in the panel, MHC isoforms (IIa, IIx, IIb, I) from a protein stained gel from the GG muscle. In the next four lanes, Western blots of the four MHC isoforms in the GG muscle stained with monoclonal antibodies SC-71 for type IIa, 6H1 for type IIx, BF-F3 for type IIb, and BA-D5 for type I. Arrow indicates the small percentage of type I found in the GG muscle. In the final column of the panel, Western blot of MHC isoform type I in the soleus muscle stained with BA-D5.