Fit a sterile 3-way stopcock with a sterile 3 mL sample syringe (syringe #1) and 5 mL waste syringe (syringe #2), primed with normal saline
Connect the apparatus to the catheter via a needless hub aseptically; the catheter valves should be set to the “off” position
Connect a sterile 5 mL syringe (syringe #3) to the third and inline end of the 3-way stopcock for normal saline flush. Syringe should be pre-filled with 5 mL normal saline
Open the distal stopcock value to syringe #2
Draw back mL of blood and then return to the patient over 3-5 seconds. Repeat 4 times. Do not return blood if there is any resistance or clots noted in the syringe.
Close the stopcock valve on syringe #2 and open the valve to syringe #3
Infuse 2 mL of normal saline through syringe #3
Close stopcock valve on syringe #3 and open the valve to syringe #2
Draw back 5 mL of blood and hold in syringe #2
Close stopcock valve on syringe #2 and open the valve to syringe #1
Draw 2.5 mL of blood into syringe #1 as the final sample
Close stopcock valve on syringe #1 and open the valve to syringe #2
Return the blood in syringe #2 back to the patient
Close stopcock valve on syringe #2 and open the valve to syringe #3
Infuse the remaining 3 mL of normal saline through syringe #3
Remove syringe #1 and transfer blood sample into a green top (sodium heparin) blood collection tube