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. 2010 Dec 1;99(11):3599–3608. doi: 10.1016/j.bpj.2010.10.018

Figure 3.

Figure 3

Effect of Trp substitutions on voltage dependence and current amplitude in the absence and presence of KCNE1. (A) Sample G-V relationships or currents from three different representative Trp mutations that affect KCNE1 modulation. Currents were elicited by depolarizing from −80 to 60 mV in 10 mV increments for 5 s. Scale: R6W 4 μA, R6W+KCNE1 1 μA. In the G-V plots, black curves are fittings of the Boltzmann equation to the mutants and the gray curves are G-V relations of WT Kv7.1 (left) and Kv7.1+KCNE1 (right). Right, Western blot probing for Kv7.1 and Gβ in the whole cell lysate and biotinylated membrane fraction from oocytes. (B) Shift in the voltage of half-maximal activation for Trp mutations in S4 relative to WT Kv7.1 in the absence or presence of KCNE1, respectively. Green identifies mutations that express low currents only in the presence of KCNE1 (M238, L239, H5, D242, R6). Blue identifies mutations that shift voltage dependence in opposing directions with and without the presence of KCNE1 (I235, R4). Pink identifies mutations that shift voltage dependence in either in the presence or absence of KCNE1 (V221, A223, A226, V241).