Table 1.

Effect of mutations in EF-Tu on the experimentally determined rate constants of EF-Tu interaction with EF-Ts and guanine nucleotides

Rate constant∗	WT†	WT‡	D109A†	E152A†/§
k1 (× 106 M−1s−1)	2.8 ± 0.1	2.0 ± 0.5	3.3 ± 0.1	2.0§
k−1 (s−1)	0.002 ± 0.001	0.002 ± 0.001	0.004 ± 0.001	0.003§
k2 (× 107 M−1s−1)	1.3 ± 0.1	1 ± 0.2	0.3 ± 0.1	0.08 ± 0.01†
k−2 (× s−1)	0.01	0.03	0.04	0.01§
k3 (× 107 M−1s−1)	3 ± 1	6 ± 1	4 ± 1	0.46§
k−3 (s−1)	438 ± 50	350 ± 50	408 ± 64	170 ± 30§
k4 (× 106 M−1s−1)	7 ± 1	14 ± 5	7 ± 1	7.6§
k−4 (s−1)	219 ± 25	125 ± 25	16 ± 1	9 ± 2§
k5 (× 105 M−1s−1)	4.3 ± 0.1	5.0 ± 1	3.9 ± 0.1	2.5 ± 0.1†
k−5 (s−1)	0.02 ± 0.01	0.03 ± 0.01	0.05 ± 0.01	0.05 ± 0.01†
k6 (× 107 M−1s−1)	12.4 ± 0.3	3 ± 0.5	3.3 ± 0.2	5.3 ± 1.6†
k−6 (s−1)	60 ± 10	60 ± 10	90 ± 10	390 ± 90†
k7 (× 106 M−1s−1)	3.9 ± 0.7	6 ± 1	1.8 ± 0.2	5.9 ± 2.7†
k−7 (s−1)	95 ± 10	85 ± 10	50 ± 5	8.5 ± 1†

^∗According to the mechanism of Scheme 1.

^†This study.

^‡Gromadski et al. (2).

^§Wieden et al. (4).