Fig. 6.

Time-dependent expression of cytokine receptors on HARA-B cells. a Immunostaining of cytokine receptor and receptor subunit (IL-6Rα, gp130, TNFRtI and IL-1RtI) on HARA-B cells with or without co-culture with astrocytes for 24, 48, and 72 h. HARA-B cells were also immunostained with anti-cytokeratin (CK) antibody. b Quantification of fluorescent intensity for each receptor or receptor subunit per area of single cell. Data were given as the percentage of intensity in control HARA-B cells without co-culture (100%). Each value represents the mean ± SEM (n = 8). ** P < 0.01, ^## P < 0.01