. 2010 Oct 17;28(1):13–25. doi: 10.1007/s10585-010-9354-8

Table 1.

Gene-specific primer pairs for real-time RT–PCR

	Primer sequence (5′–3′)		Length (bp)
Mouse
β-actin	Sense	ACCAACTGGGACGACATGGAG	380
β-actin	Antisense	GTGGTG GTGAAG CTGTAGCC	380
IL-6	Sense	ACAAGTCGGAGGCTTAATTACACAT	79
IL-6	Antisense	AATCAG AATTG CCATTGCACAA	79
IL-1β	Sense	CTCCATGAG CTTTGTACAAG G	240
IL-1β	Antisense	TG CTG ATGTACCAGTTG GG G	240
TNF-α	Sense	ATGAGCACAGAAAGCATGATCCGC	692
TNF-α	Antisense	CCAAAGTAGACCTG CCCG GACTC	692
TGF-β1	Sense	GAG AG CCCTGG GATACCAACTACTG	173
TGF-β1	Antisense	GTGTGTCCAGG CTCCAAATGTAG	173
EGF	Sense	TTTTGCCTCAGAAG GAGTGG	150
EGF	Antisense	GG CCACACTTG G CAGTATATC	150
IGF-I	Sense	GGACCAGAGACCCTTTGCGGGG	209
IGF-I	Antisense	GG CTG CTTTTGTAGG CTTCAGTG G	209
PDGF-B	Sense	TGAAATGCTGAGCGACCAC	137
PDGF-B	Antisense	AGCTTTCCAACTCGACTCC	137
Human
β-actin	Sense	ATG GCCACG GCTGCTTCCAG C	237
β-actin	Antisense	CATGGTGGTGACAGACCG CCG	237
IL-6Ra	Sense	CATTGCCATTGTTCTGAGGTT	271
IL-6Ra	Antisense	AGTAGTCTGTATTG CTGATGT	271
gpi30	Sense	TGGAGTGAAGAAGCAAGTGG	303
gpi30	Antisense	AACAGCTGCATCTGATTTGC	303
TNFRI	Sense	TG CCTACCCCAGATTG AG AA	121
TNFRI	Antisense	ATTTCCCACAAACAATGGAGTAG	121
IL-1 Rtl	Sense	AAG GTG GAG G ATTCAGG ACAT	284
IL-1 Rtl	Antisense	AG CCTATCTTTGACTCCACTA	284
IL-1 ra	Sense	CAGAAGACCTCCTGTCCTATGAGG	424
IL-1 ra	Antisense	GCTGTGCAGAGGAACCA	424