Figure 1.
Basal and LCA-induced expression of antioxidant and transporter proteins was suppressed in Nrf2 disrupted mice. (A) Representative immunoblot images of cytoprotective and antioxidant proteins (50 µg per lane) for Nrf2+/+ and Nrf2−/− mice administered vehicle corn oil or LCA (125 mg·kg−1 body wt. twice daily for 4 days). (B) Densitometric measurement of protein abundance of gel images from (A). Repeated analysis of other liver samples yielded similar results. β-Actin was used as equal protein loading control and denominator adjuster for all target proteins; mean values of two representative images. Gclc, glutamate cysteine ligase catalytic subunit; Ho1, haeme oxygenase-1; LCA, lithocholic acid; Mrp, multidrug resistance-associated protein; Nrf2, nuclear factor (erythroid 2-related) factor 2; Trx1, thioredoxin reductase 1.