Table 1.

Candidate age-grading genes selected from the microarray analysis

Transcript identifier	Gene name	Putative function
AGAP004115-RA		Cystinosin, putative
AGAP006187-RA	G12_ANOGA	Protein G12 precursor (ANG12)
AGAP006985-RA		Conserved hypothetical protein
AGAP010398-RA		Dimethylaniline monooxygenase, putative
AGAP012936-RA		Cystinosin, putative
AGAP006829-RA	CPR59	Cuticular protein 59
AGAP007963-RA*		Calcium-binding protein, putative
AGAP008447-RA†	CPLCG4	Adult cuticle protein
AGAP009871-RA*	CPR75	Cuticular protein 75 (RR-1 family)
AGAP010592-RA	RS7_ANOGA	40S ribosomal protein S7

Transcript identifiers and gene function originate from Vectorbase annotations. When gene function has not been annotated putative gene function has been inferred from Drosophila melanogaster and/or Aedes aegypti orthologues. The four best performing gene transcripts used for the age estimation analysis shown in Fig. 3 are in bold.

^*Orthologues of genes selected for use in the Aedes aegypti age estimation assay (Cook et al., 2006).

^†Gene excluded from the analysis because of possible confounding effects of insecticide resistance status (see Discussion).