Figure 2.

(a) Distributions of sedimentation coefficients c(s) derived from sedimentation velocity data for wild-type Vpu_1–40 at loading concentrations of 52 μM with 40 mM C₈E₅ (red line), 100 μM with 40 mM C₈E₅ (blue line), and 130 μM with 10 mM C₈E₅ (green line). A₂₈₀ is the optical absorbance at 280 nm. (b) Distributions for Y29T-Vpu_1–40 at loading concentrations of 54 μM with 40 mM C₈E₅ (red line), 107 μM with 40 mM C₈E₅ (blue line), and 137 μM with 10 mM C₈E₅ (green line). Data were collected at 50 krpm and 20.0°C and analyzed with SEDFIT software. [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com.]