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S-75 gel filtration of sRz and sRz1. (A) Elution profile of sRz (•). (B) Elution profiles of SUMOφsRz1 (•) and SUMOφsRz1 ULPI cleavage reaction (▪). S-75 standards are indicated by arrow heads, from left to right: 75, 44, 29, 13.7, and 6.5 kDa.
