Fig. 2.

PCR analysis of restriction digestion and ligation efficiency used for the evaluation of 3C template (A) To determine digestion efficiency, primer pairs D1/D2 and D3/D4 were used. When the HindIII restriction digestion was completed, D1/D2 primer pair gives a faint PCR band whereas D3/D4 primers generate a band whether DNA templates are undigested (-) and digested (+) with HindIII. (B) To determine the efficiency of self ligation, the primer L1/L2 was used. DNA was prepared before (-) and after (+) the ligation step. The 3C templates from Day 3 of RA treatment were also tested with same ways and obtained similar results (Data not shown).