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. Author manuscript; available in PMC: 2010 Dec 8.
Published in final edited form as: Sci Signal. 2009 Nov 10;2(96):ra72. doi: 10.1126/scisignal.2000464

Fig. 5.

Fig. 5

Sulfhydration enhances actin polymerization in vitro and in HEK293 cells. (A) Actin is sulfhydrated in vitro with 200 μM NaHS for 30 min at 37°C, an effect reversed by DTT (200 μM for 10 min). (B) NaHS (100 μM for 30 min at 37°C) sulfhydrates actin in HEK293 cells. (C) Immunocytochemical analysis of HEK293 cells treated with 100 μM NaHS for 1 hour at 37°C reveals rearrangement of the actin cytoskeleton (arrows show the extension of thin processes containing actin filaments in individual NaHS treated cells). Actin is stained in red. Scale bars, 20 μm. (D) NaHS (200 μM) enhances actin polymerization in vitro, an effect reversed by DTT (200 μM). (E) NaHS (200 μM) has no effect on actin depolymerization.