Skip to main content
. Author manuscript; available in PMC: 2011 Apr 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2010 Jan 7;30(4):694–701. doi: 10.1161/ATVBAHA.109.200741

Figure 2. Csrp1-null mice are viable and have a grossly-normal vasculature.

Figure 2

(A) Analysis of genotypes from Csrp1 heterozygous intercross. Expected mendelian ratios were seen for the three genotypes. (B–E) Histological analysis of aortic sections from wildtype (+/+) and Csrp1-null (−/−) mice. Sections in panels B and C are stained with Verhoff’s stain to delineate the elastin fibers between the smooth muscle in the medial region of the vessel. Sections in D and E are stained with Trichrome; the smooth muscle layers label red, alternating with the blue labeling of the elastin fibrils. (F–H) Wild-type (+/+) and (F′–H′) Csrp1-null (−/−) aortic smooth muscle cells labeled with antibodies indicated. Antibodies used for immunodetection: anti-CRP1 (F,F′); anti-smooth muscle α-actin (G,G′), and anti-vinculin (H,H′). Note lack of labeling with anti-CRP1 serum in cells derived from Csrp1-null mice. (scale bar in F=100 μm.)