FIG. 2.

Intracellular distribution of MacroH2A following nuclear transfer. Enucleated metaphase II oocytes (n = 25) were injected with cumulus cell nuclei and observed by indirect immunofluorescence at 10 min (top row), 1 h (middle row), and 3 h (bottom row) postinjection. Injected oocytes were stained with a MacroH2A-specific antibody (left column) and propidium iodide (PI) to visualize DNA (middle column). Green (MacroH2A) and red (PI) signals were merged to show colocalization (right column). Representative images are shown.