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. 2010 Sep 23;21(1):6–12. doi: 10.1093/glycob/cwq144

Fig. 2.

Fig. 2.

Galectin-1 constructs share binding specificities. Biotinylated galectin-1, gal-1GG, gal-1-9-1 and gal-1L9 were assayed on a glycan microarray. (A) Galectin-1 constructs bind specifically to LacNAc, either alone or on a core 2 O-glycan. Binding, assayed by relative fluorescence units (RFUs), are shown for glycans 131 (Core 1, Galb1-3GalNAca-Sp8), 182 [Core 2 base, GlcNAcb1-6(Galb1-3)GalNAca-Sp8], 157 [Core 2, Galb1-4GlcNAc1-6(Galb1-3)GalNAca-Sp8] and 161 (LacNAc, Galb1-4GlcNAc-Sp0). Data are mean RFU ± SE of six replicate assays. (B) Galectin-1 constructs bind to biantennary N-glycans; binding is reduced on single-chain or α2,6-sialylated N-glycans. Binding in RFU is shown for glycans 161 (LacNAc, Galb1-4GlcNAc-Sp0), 50 (N-asialo, Galb1-4GlcNAcb1-2Mana1-3(Galb1-4GlcNAcb1-2Mana1-6)Manb1-4GlcNAcb1-4GlcNAcb-Sp12), 360 [N-single chain, Mana1-3(Galb1-4GlcNAcb1-2Mana1-6)Manb1-4GlcNAcb1-4GlcNAcb-Sp12], and 314 [N-single α2,6, Neu5Aca2-6Galb1-4GlcNAcb1-2Mana1-3(Galb1-4GlcNAcb1-2Mana1-6)Manb1-4GlcNAcb1-4GlcNAcb-Sp12], relative to LacNAc (glycan 161). Data are mean RFU over LacNAc RFU ± S.E. of six replicate assays.