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. 2010 Apr;12(2):117–125. doi: 10.1089/cell.2009.0077

FIG. 1.

FIG. 1.

Induction of iPS cells from cultured human amniotic fluid (AF) cells. (a) Time course of the generation of iPS colonies from AF cells (AF), neonatal (BJ), and adult skin fibroblasts (Skin). (b) Frequency of AF-iPS, BJ-iPS, and iPS colonies from adult skin generated 28 days after infection. (c, d, e). Morphology of cultured AF cells (c), BJ fibroblasts (d), and primary skin fibroblasts (e). (f, g, h, j) Morphology of an established AF-iPS colony feeder dependent (f) or feeder independent plated onto Matrigel (g), BJ-iPS colonies (h), iPS cells derived from adult skin (i), Typical image of the HES-2 human ES cell line (j). (k, l, m, n, o) Immunohistochemistry of selected AF-iPS colonies. Cells were stained with alkaline phoshatase (k) OCT 3/4 (l), SOX2 (m), SSEA4 (n), and NANOG (o).