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. Author manuscript; available in PMC: 2011 Oct 1.
Published in final edited form as: Cancer Res. 2010 Sep 28;70(19):7543–7552. doi: 10.1158/0008-5472.CAN-09-4582

Figure 2. UPLC-MS-MS analysis of nicotine and its metabolites in the urine of smokers.

Figure 2

Shown are representative chromatograms for each analyte and a representative deuterated standard [nicotine (methyl-D3)- Gluc)] that was identified simultaneously from the MRM analysis of a single urine sample as described in the Materials and Methods. For each compound, the ratio of analyte peak area to that of the deuterated standard was used for quantification as described. The m/z transition monitored for each analyte is detailed on each graph.