Figure 2.

Processive polymerization. (a) In the presence of ADP the exosome moves away from the junction via polymerization, decreasing FRET. (b) A representative trace shows how the polymerization time is measured. A time interval was determined between when FRET starts to decrease and when it saturates to the lowest value. (c) Representative traces show that polymerization reaction slows down at lower ADP concentrations. (d) The velocity of polymerization vs ADP concentration and a Michaelis−Menten fit. Error bars denote standard error of mean.