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. Author manuscript; available in PMC: 2010 Dec 8.
Published in final edited form as: Nat Immunol. 2008 Nov 9;9(12):1347–1355. doi: 10.1038/ni.1677

Figure 1. IL-4 inhibits TGF-β–induced Foxp3+ T cells.

Figure 1

(a–e) Naïve CD4+Foxp3− CD62L+ T cells from Foxp3-GFP mice activated with or without cytokines in the presence of irradiated syngenic APCs with 1 μg/ml anti-CD3. (a) Flow cytometry for GFP expression in CD4+ T cells 72h after activation in the presence of TGF-β, IL-4 or both. Data represent one of three experiments. (b) ELISA assay or bead array for the indicated cytokines in 48 h culture supernatants. Data represent one of two experiments. (c) Flow cytometry for intracellular expression of IL-10, IL-4, IL-17 and IFN-γ in CD4+ T cells measured at day 4 post-activation. Data represent one of three experiments. (d) Proliferation of cytokine-treated WT T cells was measured by 3H-thymidine incorporation in triplicate wells of T cells activated in the presence of irradiated syngenic APCs with 1 μg/ml anti-CD3. Proliferation was assessed in triplicate wells and is presented as mean ± s.d. Data represent one of three experiments. (e) Quantitative RT-PCR for the indicated transcripts for various transcription factors (mRNA expression relative to Gapdh). Data represent mean one of two experiments.