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. 2003 Dec 1;100(25):15053–15058. doi: 10.1073/pnas.2433717100

Fig. 4.

Fig. 4.

Ex vivo expansion of effector and memory cell populations. (A) Expansion of young (filled bars) and aged (open bars) primary effector populations after 4 days of culture (Left) and memory effector populations after 3 days of culture (Right). Primary effector cultures were generated under the appropriate conditions for each effector population; memory cultures were stimulated with Ag/APC alone. *, P < 0.05, statistically significant difference by Student's t test; n = 4. (B) Th2 memory cells generated from young and aged naive cells were recovered 4 weeks after transfer, CFSE-labeled, and cultured with Ag/APC alone or with added IL-2 for 3 days. Histograms are gated on Tg (Vβ3+) CD4 cells and show CFSE profiles and CD25 and CD69 expression of young (open histograms) and aged (shaded histograms) memory cells; dotted lines represent isotype controls. The results are representative of three experiments.