Fig. 1.

MED1 thymine glycosylase activity for O⁶–meG:T mismatches. Double-stranded oligonucleotides, bearing O⁶–meG:T mismatches in a CpG or GpG context and ³²P-labeled at the 3′ end on the bottom strand, were treated with purified recombinant MED1 protein at 37°C. The reactions were then treated with NaOH at 90°C, to cleave the sugar-phosphate backbone at the abasic site, and were separated by PAGE. OG: O⁶–meG. Lanes 1 is a positive control.