Fig. 5.
Tolerance of Med1-deficient cells to alkylation damage. (A) Cell cycle analysis by flow cytometry of Med1+/+ and Med1-/- MEF treated with 0, 0.5 and 1 μg/ml MNNG for 48 and 72 h, and stained with propidium iodide. Each profile represents the analysis of 20,000 events. Yellow boxes mark the subG1 region with the indicated percent of apoptotic cells. (B) Western blot analysis with anti-phospho-p53 Ser-15 antibody of lysates from MEF treated or not with 0.5 μg/ml MNNG for 24–72 h. The asterisk marks a cross-reacting band. (C) Quantification of the anti-phospho-p53 Ser-15 Western blot (B). Relative units of p53 activation are computed with respect to untreated cells at the same time point.