Fig. 2.

Induction of apoptosis by rapamycin in ALL cell line T309. Eμ-ret transgenic mouse-derived pro-B cell line T309 was treated with increasing concentrations of rapamycin (rap) for 3 days. Cells were assessed for apoptotic response by labeling with FITC-conjugated annexin V. Percentages of apoptotic cells are indicated. Flow cytometric histograms show untreated (a), 1 ng/ml rapamycin (b), and 100 ng/ml rapamycin (c). Peak on the right in a-c represents FITC-conjugated annexin V-positive cells.