Fig. 2.

Sensorgrams of immobilized ORFV NZ2 CBP binding chemokines. Sensorgrams are plotted as the mass of protein binding (in RU) to immobilized ORFV NZ2 CBP as a function of time. Experimentally derived curves (black lines) from three repeat injections of MCP-1 (A), MIP-1α (B), MCP-3 (C), or eotaxin (D) at various concentrations (1, 3, 9, and 27 nM, bottom line to top line) are shown overlaid. Sensorgram on right in D shows the extended dissociation of eotaxin from the ORFV CBP. Triplicate curves were globally fitted with bia-evaluation 3.1 software using a 1:1 mass transport model (red lines) to determine the kinetic parameters presented in Table 1.