Table 1. GFP-myosin Vb tail expression inhibits RSV replication and redirects budding to the basolateral cell surface.
Total virus yield, log10 pfu/ml*
|
% Apical RSV‡
|
||||
---|---|---|---|---|---|
Route of infection | + Dox. | – Dox. | Fold reduction† | + Dox. | – Dox. |
Apical | 4.2 | 1.6 | 398 | 97 | 24 |
Basolateral | 5.2 | 4.7 | 3 | >99 | 10 |
Total virus yield was calculated by separately determining the titer of virus released into the apical or basolateral supernatants (pfu/ml), adding those values, and converting the total to a log10 pfu/ml value
Fold reduction was calculated by dividing the virus titer (pfu/ml) in the presence of doxycycline (Dox.) by that in the absence of doxycycline
Percentage of apical RSV was calculated by dividing the titer of virus recovered from the apical supernatant (pfu/ml) by the combined apical and basolateral supernatant titers (pfu/ml) and multiplying the quotient by 100