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Immunoprecipitation analysis. Soluble membrane extracts of C. trachomatis and C. pneumoniae preincubated with native Apo-B containing lipoproteins were immunoprecipitated with Apo-B specific polyclonal antibody and subjected to SDS PAGE. Immunoprecipitated (IP) supernatant and pellet were obtained as described in Materials and Methods. Membranes were immunoblotted with MOMP-specific monoclonal antibodies.
