Fig. 2.

Desensitization of TPRM5 is Ca²⁺-dependent. (a) Responses in an excised patch (-80 mV) to repeated applications of Ca²⁺. Note that the current declined gradually in response to repeated exposures to 40 μM Ca²⁺ but declined dramatically in response to 500 μM Ca²⁺. (b) Response in patches to prolonged exposure to Ca²⁺ (-80 mV). Single exponential fits are shown in red. (c) The rate of decay of the current (1/τ) is linearly related to Ca²⁺ concentration, over the range measured, indicating that desensitization is Ca²⁺-dependent (mean ± SEM, n = 5 patches for each data point).