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Tumor-derived neurospheres give rise to neurons that proliferate aberrantly. (A) Clonal neurosphere derived from the BT4 tumor, double-labeled for β-III-tubulin (red) and Ki-67 (green). Nuclei were counterstained with DAPI (blue). (B) Double-label for BrdUrd (green), visualized after a 14-h pulse and TuJ1 (red). (Scale bar in B = 15 μm in A and B.)
