Figure 3.
Phagocytosis of borrelia by DAE15 and IDE12 cells. Cell layers were incubated with hkFITC or viable JMNT borreliae for 24 hours prior to fixation and examination. Cells containing internalized spirochetes with a coiled appearance were considered phagocytic. Phagocytosis of borrelia by uninfected cells (black bars) and Rickettsia peacockii-infected cells (grey) were compared uninfected cells treated with cytochalasin B (crosshatched) and R. peacockii-infected cells treated with cytochalasin B (white) cultures. Asterisks indicated statistically significant differences between samples. A. Phagocytosis of dead Borrelia burgdorferi by DAE15 and IDE12 cells. B. Phagocytosis of live borreliae by DAE15 and IDE12 cells.