Skip to main content
. Author manuscript; available in PMC: 2010 Dec 8.
Published in final edited form as: Neuron. 2009 Nov 12;64(3):381–390. doi: 10.1016/j.neuron.2009.08.035

Figure 5. Exocytosis of endogenous AMPARs detected by two-photon glutamate uncaging-evoked responses on dendrites.

Figure 5

(A) Example of structural changes following glutamate uncaging-evoked LTP; glutamate uncaging-mediated responses obtained before and after LTP at locations indicated by the yellow filled circles. (B) Example of AMPAR-mediated currents in the spine and dendrite before and after glutamate uncaging-evoked LTP. Right panel shows response traces (average of 3 responses). (C) Mean changes in AMPAR-mediated inward currents in spines (n = 10, 10 cells) and dendrites (n = 17, 17 cells) following glutamate uncaging-evoked LTP. Black points indicate dendrite responses obtained in the absence of the LTP induction protocol (n = 7, 7 cells, *p < 0.05). Each point is an average of 3 responses obtained every 20 sec. (D) Correlation between changes in spine volume and spine responses (n = 9, 9 cells, r = 0.87, **p < 0.01). (E) Significant correlation between changes in spine responses and changes in dendrite responses at 10 min (n = 10, 10 cells, r = 0.69, *p < 0.05) but not at 1 min after LTP induction (n = 10, 10 cells, p = 0.16).