NF-κB target genes are repressed by both hPR-A and hPR-B. A, T47D-C42 cells, stably expressing lacZ, hPR-A, or hPR-B, or T47D-A18 cells were treated with vehicle or 10 nm R5020 (R) in the presence of 10 ng/ml IL-1β (I) for 6 h, followed by qPCR analysis of mRNA levels. Results are expressed as percent activation compared with IL-1β- and vehicle-treated cells. B, Immunoblotting of the protein levels of PR in whole-cell extracts from T47D-C42 cells or T47D-A18 cells with α-tubulin as a loading control.