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. 2010 Oct 27;24(12):2292–2302. doi: 10.1210/me.2010-0289

Figure 5.

Figure 5

Effects of progestins and antiprogestins on IL-1β-induced activation of cytokine genes. A, T47D-A18 cells were cotreated with 10 ng/ml IL-1β (I) and vehicle (v), 10 nm R5020 (R), 100 nm RU486 (RU), or 1 μm ZK98299 (ZK) for 6 h. mRNA levels of CCL2, CCL20, IL-8, and CCL4 were quantitated using qPCR. Results are expressed as relative expression over IL-1β- and vehicle-treated cells. B, Serum-starved T47D-A18 cells were treated with vehicle, 10 nm R5020, 100 nm RU486 (RU), or 1 μm ZK98299 (ZK) in the presence or absence of 10 ng/ml IL-1β for 2 h. ChIP was performed with either mouse IgG control or PR1294 antibody. qPCR analysis was performed using primers spanning a region in the NF-κB-binding region of CCL20 or IL-8. The results are presented as percent input.