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. 2010 Oct 27;24(12):2292–2302. doi: 10.1210/me.2010-0289

Figure 6.

Figure 6

Use of CBI to probe the role of AF-2 in PR-mediated transcriptional repression. A, T47D-A18 cells were infected with a Gal4-DBD-LX23 or Gal4-DBD-LXAA (control) expressing adenovirus. Viruses were removed 2 h after infection, and 42 h later, cells were treated with vehicle (v) or 10 nm R5020 (R) in the presence of 10 ng/ml IL-1β (I) for 6 h. mRNA levels of CCL2, CCL20, IL-8, and CCL4 were quantitated using qPCR. Results are expressed as relative expression compared with that observed in Gal4-LXAA-infected and IL-1β- and vehicle-treated cells. B, Western blot analysis of the expression of the Gal4-DBD fused proteins in whole-cell extracts from adenovirus-infected cells with GAPDH as a loading control.