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. 2010 Oct 13;24(12):2281–2291. doi: 10.1210/me.2010-0133

Figure 3.

Figure 3

Dax1 binds to and up-regulates Oct4 transcription through the LRH-1 proximal promoter site. A, ChIP was performed in mES cells as described in Materials and Methods. Immunoprecipitation (IP) was performed with normal serum or anti-Dax1 antibody, and QPCR was carried out with primer sets flanking the Oct4 PP LRH-1 site, proximal enhancer site (PE), or control site. Results are shown from independent experiments quantitated by either QPCR or PCR and agarose gel analysis. *, P < 0.02; **, P < 0.006. B, mES cells were transfected with either 200 ng wild-type (wt) Oct4-Luc reporter or reporter in which the LRH-1 PP site is mutated. Cells were cotransfected with 200 ng empty vector or Dax1 and 48 h later were harvested and luciferase assay was carried out.