Figure 3.

Induction of transgenic stc1 with and without heat shock (HS) assayed by qPCR. Both endogenous transcript (stc1) and transgene-derived transcript (stc1T) are assayed. Induction of the transgene is observed in Tg(hsp70::stc1-IRES-EGFP) embryos 60 min after heat shock of 5-dpf larvae (means ± se). Absolute expression levels were scaled to those for β-actin. Endogenous stc1 levels were assayed with primers targeting the 3′-untranslated region, whereas transgenic stc1 were assayed with primers targeting the 3′-coding sequence and the IRES sequence of the transgene. stc1T transcript levels in wild-type sibling larvae and transgenic larvae without heat shock are comparable to those observed with no-template qPCR controls (data not shown).