Figure 7.
fgf23 up-regulation in trpm7 mutants and effect of knockdown on kidney stone incidence. A, fgf23 is weakly expressed expressed in CS (arrow, A) of wild-type (wt) larvae but is strongly up-regulated in trpm7 mutant siblings (A′) at 4 dpf. (Different orientation of CS from that shown in Fig. 2B is not unusual at this stage.) B, trpm7 mutants injected with fgf-sb1 morpholino are not morphologically distinguishable from uninjected siblings. C, fgf23 knockdown in trpm7 mutants reduces the incidence of kidney stones compared with uninjected controls (χ2 = 11.0; P < 0.001), whereas stc1 knockdown has no effect. Values are least squares means ± se, normalized to the incidence of kidney stones in uninjected trpm7 mutant siblings.