Figure 1.
mRNA expression of PPARG, NR1H2, NR1H3, ABCA1, and ABCG1 during luteinization in vivo. Follicles were aspirated before (0) or up to 24 h after an ovulatory bolus of hCG was administered to rhesus macaques undergoing COS protocols. Granulosa cells were processed for RNA and real time RT-PCR analysis for (A) PPARG, (B) NR1H3, (C) NR1H2, (D) ABCA1, and (E) ABCG1 was performed. Expression levels of all genes were normalized to the internal standard RPL19 and plotted as relative mRNA levels. Time points with different lowercase letters are significantly different (P < 0.05; n = 3–5 per time point).