Figure 5.

Effect of LXR agonist treatment on mRNA expression of steroidogenic genes during in vitro luteinization. Nonluteinized granulosa cells were isolated as and treated with FSH or FSH+hCG with or without the LXR agonist T0901317 (0–10 μm) for 24 or 48 h. The mRNA expression of STAR (A and F), CYP11A1 (B and G), HSD3B2 (C and H), SCARB1 (D and I), and LDLR (E and J) was determined by real time RT-PCR. Data are presented as mean ± sem of percent change over time-matched FSH treatment. Bars with different lowercase letters are significantly different (n = 3; P < 0.05).