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. 2010 Sep 29;151(12):5851–5864. doi: 10.1210/en.2010-0350

Figure 1.

Figure 1

Aldosterone enhances IGF-I signaling in VSMCs. A, Aldosterone (10 nmol/liter) was added for the indicated times then IGF-I (50 ng/ml) for 5 min. Cell lysates were immunoblotted for pAKT, pMAPK, and β-actin. IB, Immunoblot; Aldo; aldosterone. ***, P < 0.001 when IGF-I plus aldosterone was compared with IGF-I alone. B, VSMCs were treated as in A except increasing concentrations of aldosterone (1–10 nmol/liter) were added for 18 h. *, P < 0.05, **, P < 0.01 aldosterone (2 or 10 nmol/liter) plus IGF-I compared with IGF-I alone. C, VSMCs were treated as in A, and then IGF-I (50 ng/ml) was added for the indicated times. Cell lysates were immunoblotted for pAkt and then reprobed using anti-Akt antibody. ***, P < 0.001, **,P < 0.01 IGF-I for 5 or 10 min plus aldosterone compared with IGF-I alone. D, The same experimental conditions as in C were used to detect MAPK phosphorylation. E, VSMCs were treated as in A. Cell lysates were immunoblotted for phos-p70S6K or with anti-β-actin. *, P < 0.05 (2 nmol/liter), ***, P < 0.001 (10 nmol/liter) aldosterone plus IGF-I compared with IGF-I alone. The results represent the mean ± sem scanning densitometry units from three experiments and are expressed as the ratio of the phosphorylated form divided by the total protein.