Skip to main content
. 2010 Sep 29;151(12):5851–5864. doi: 10.1210/en.2010-0350

Figure 6.

Figure 6

Aldosterone increases synthesis of IGF-IR and enhances IGF-I-mediated IGF-IR and IRS-1 phosphorylation. Aldosterone (10 nmol/liter) was added for 18 h and then IGF-I (50 ng/ml) for the indicated times. A, IGF-IR phosphorylation was determined as described in Materials and Methods. The results from three experiments are expressed as arbitrary scan units of the phosphorylated IGF-IR or as the ratio of phospho/total IGF-IR and are presented as mean ± sem. IB, Immunoblot; Aldo, aldosterone. B, VSMCs were incubated with eplerenone (10 μmol/liter) or Act-D (20 nmol/liter) for 1 h and then aldosterone (10 nmol/liter) for 18 h. The same procedures as in A were used. C, Cells were incubated for 1 h with or without anti-αVβ3 IgG (1 μg/ml) and aldosterone (10 nmol/liter) for 18 h and then IGF-I (50 ng/ml) for 5 min. IGF-IR phosphorylation was determined and expressed as in A. D, The same experimental conditions as in B were used. Cell lysates were immunoprecipitated with an anti-IRS-1 and immunoblotted with anti p-Tyr. The membranes were stripped and reprobed with an anti-IRS-1. E, The same experimental conditions as in C were used. IRS-1 phosphorylation was determined as above. **, P < 0.01; ***, P < 0.001. NS, Not significant.