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. 2010 Dec 8;5(12):e14258. doi: 10.1371/journal.pone.0014258

Figure 6. Distribution of DDX3 and IPS-1.

Figure 6

(A) DDX3 colocalizes with IPS-1 on the mitochondria in Oc cells. HA-tagged DDX3 and FLAG-tagged IPS-1 were co-transfected into Oc cells. After 24 hrs, cells were fixed with formaldehyde and stained with anti-HA polyclonal and FLAG monoclonal Abs. Alexa488 (DDX3-HA) or Alexa633 antibody was used for second antibody. Mitochondria were stained with Mitotracker Red. Similar IPS-1-DDX3 merging profiles were observed in Huh7.5.1 cells (Fig. S3). (B,C) O cells with the HCV replicon poorly formed the DDX3-IPS-1 complex. Plasmids carrying IPS-1 (100 ng) or DDX3 (150 or 300 ng) were transfected into O (HCV replicon +) as in Oc cells (no replicon, panel A). After 24 hrs, localization of IPS-1 and DDX3 was examined by confocal microscopy. Two representatives which differ from the conventional profile (as in panel A) are shown. Similar sets of experiments were performed four times to confirm the results.